CLUSTAL W (1.83) multiple sequence alignment RAF1_Hsap_RAF -----------VML-STRIGSGSFGTVYKGKWHGDVAVK-I--------LKVVDPTP--E KSR2_Hsap_KSR -----------LEI-GELIGKGRFGQVYHGRWHGEVAIR-L--------IDIERDNE--D KSR1_Hsap_KSR -----------VEL-GEPIGQGRWGRVHRGRWHGEVAIR-L--------LEMDGHNQ--D phl_Dmel_RAF -----------ILI-GPRIGSGSFGTVYRAHWHGPVAVK-T--------LNVKTPSP--A ksr_Dmel_KSR -----------LLL-LERIGQGRFGTVHRALWHGDVAVK-L--------LNEDYLQD-EH KSR2_Mmus_KSR -----------LEI-GELIGKGRFGQVYHGRWHGEVAIR-L--------IDIERDNE--D KSR1_Mmus_KSR -----------VEL-GEPIGQGRWGRVHRGRWHGEVAIR-L--------LEMDGHNQ--D BRAF_Mmus_RAF -----------ITV-GQRIGSGSFGTVYKGKWHGDVAVK-M--------LNVTAPTP--Q ARAF_Mmus_RAF -----------VQL-LKRIGTGSFGTVFRGRWHGDVAVK-V--------LKVAQPTA--E lin-45_Cele_RAF -----------FII-QYKVGSGSFGTVYRGEFFGTVAIK-K--------LNVVDPTP--S ksr-2_Cele_RAF TWEDVTIKLTDVDV-MTKIGDGRFGSVYFGGYHGNAAVR-F--------VNMNYLSQEDR ksr-1_Cele_KSR -----------FDKQAPIIGRGRFGKVLRGFHYGDVAVK-V--------YTMEHISDASK BRAF_Hsap_RAF -----------ITV-GQRIGSGSFGTVYKGKWHGDVAVK-M--------LNVTAPTP--Q ARAF_Hsap_RAF -----------VQL-LKRIGTGSFGTVFRGRWHGDVAVK-V--------LKVSQPTA--E RAF1_Mmus_RAF -----------VML-STRIGSGSFGTVYKGKWHGDVAVK-I--------LKVVDPTP--E KSR1_Spur_KSR ------------------------------------------------------------ Raf_Spur_RAF -----------IRL-GSRIGAGSFGTVFSGQWHGSVAVK-R--------LNVKDPTP--S KSR2_Spur_KSR -----------LKY-GRCIEKGKLGSTYKGHWHGEVLIQ-T--------R---YSKS--T AqueK675_Aque_KSR -------------------------------------------------------RQ--E AqueK676_Aque_KSR -----------LKL-LEKLGSGPVAEVYKGYWHGEVAVK-R--------FVLPNATP--K AqueK677_Aque_RAF -----------IEV-GERIGSGSFGTVYKGKWHGPVAVK-K--------LNVSNPTE--Q AqueK678_Aque_RAF -----------IEV-GERIGSGSFGTVYKGKWHGPVAVK-K--------LNVSNPTE--Q AqueK679_Aque_RAF -----------TIL-KETLGNGAYGVVYKAKCDELICAAKYMHPIFFALSEDPAV--NVV RAF1_Hsap_RAF QFQAFRNEV-AVLRKTRHVNILLFMGYM-TK---DNLAIVTQWCEGS-SLYKHLHVQ-ET KSR2_Hsap_KSR QLKAFKREV-MAYRQTRHENVVLFMGACMSP---PHLAIITSLCKGR-TLYSVVRDA-KI KSR1_Hsap_KSR HLKLFKKEV-MNYRQTRHENVVLFMGACMNP---PHLAIITSFCKGR-TLHSFVRDP-KT phl_Dmel_RAF QLQAFKNEV-AMLKKTRHCNILLFMGCV-SK---PSLAIVTQWCEGS-SLYKHVHVS-ET ksr_Dmel_KSR MLETFRSEV-ANFKNTRHENLVLFMGACMNP---PYLAIVTSLCKGN-TLYTYIHQR-RE KSR2_Mmus_KSR QLKAFKREV-MAYRQTRHENVVLFMGACMSP---PHLAIITSLCKGR-TLYSVVRDA-KI KSR1_Mmus_KSR HLKLFKKEV-MNYRQTRHENVVLFMGACMNP---PHLAIITSFCKGR-TLHSFVRDP-KT BRAF_Mmus_RAF QLQAFKNEV-GVLRKTRHVNILLFMGYS-TK---PQLAIVTQWCEGS-SLYHHLHII-ET ARAF_Mmus_RAF QAQAFKNEM-QVLRKTRHVNILLFMGFM-TR---PGFAIITQWCEGS-SLYHHLHVA-DT lin-45_Cele_RAF QMAAFKNEV-AVLKKTRHLNVLLFMGWV-RE---PEIAIITQWCEGS-SLYRHIHVQ-EP ksr-2_Cele_RAF RADVFATEIVSAykNSRHDHIALFYGYVSDP-VTNTYAIVTNFYQHN-TLYHRIHEQLSE ksr-1_Cele_KSR KAEEFKLEV-SAYKNTRHDNIALFLGYFMSD---GQYGMVMSLSKGSQSLYTLLHVV-RE BRAF_Hsap_RAF QLQAFKNEV-GVLRKTRHVNILLFMGYS-TK---PQLAIVTQWCEGS-SLYHHLHII-ET ARAF_Hsap_RAF QAQAFKNEM-QVLRKTRHVNILLFMGFM-TR---PGFAIITQWCEGS-SLYHHLHVA-DT RAF1_Mmus_RAF QLQAFRNEV-AVLRKTRHVNILLFMGYM-TK---DNLAIVTQWCEGS-SLYKHLHVQ-ET KSR1_Spur_KSR ------------------------------------------LCKGQ-TLYTHIHLR-KD Raf_Spur_RAF QLQAFKNEV-AVLRKTRHANILLFMGCT-SK---PQLAIVTQWCEGS-SLYKHLHVL-DT KSR2_Spur_KSR DINDFLDEV-AMLSKIRHENIALFMGACVEP---GNLAVVNSIHKGP-SLYHHLHVQ-RR AqueK675_Aque_KSR DVRSFANEV-NTLCCIRHENIQLFMGVCLDLPAGDTFGIIMSEVKGD-TLYNHIHKL-GH AqueK676_Aque_KSR QINKFREEV-AVLKKIRHENLALFMGVCLTT---PNLAIITSYCKGY-TLYKLLHYW-SE AqueK677_Aque_RAF QMQAFKNEV-AVLMKTRHANILLFMGWT-SK---PRLTIVTQWCDGS-TLFSHIHVL-ED AqueK678_Aque_RAF QMQAFKNEV-AVLMKTRHANILLFMGWT-SK---PRLTIVTQWCDGS-TLFSHIHVL-ED AqueK679_Aque_RAF ATDKFKKEC-QLLSRLKHPNIVQYLGTH-EVNGI-DTVVLLMELMDS-SLHAYLHAHVDS :*. :: RAF1_Hsap_RAF --KFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE---GL-TVKIGDFGLATV KSR2_Hsap_KSR --VLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDN----G-KVVITDFGLFSI KSR1_Hsap_KSR --SLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDN----G-KVVITDFGLFGI phl_Dmel_RAF --KFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHE---DL-SVKIGDFGLATA ksr_Dmel_KSR --KFAMNRTLLIAQQIAQGMGYLHAREIIHKDLRTKNIFIEN----G-KVIITDFGLFSS KSR2_Mmus_KSR --VLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDN----G-KVVITDFGLFSI KSR1_Mmus_KSR --SLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDN----G-KVVITDFGLFGI BRAF_Mmus_RAF --KFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHE---DL-TVKIGDFGLATV ARAF_Mmus_RAF --RFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHE---GL-TVKIGDFGLATV lin-45_Cele_RAF RVEFEMGAIIDILKQVSLGMNYLHSKNIIHRDLKTNNIFLMD---DMSTVKIGDFGLATV ksr-2_Cele_RAF --DFDQSWTFQISLQICQAMSYLHKKKILHRDLRTKNILLDN---PN-RVVVTDFALMKL ksr-1_Cele_KSR --KLDLATTRKIAQQICQAVSYLHTKKILHKDLRSKNILLES---KN-KVVITDFGILSM BRAF_Hsap_RAF --KFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHE---DL-TVKIGDFGLATV ARAF_Hsap_RAF --RFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHE---GL-TVKIGDFGLATV RAF1_Mmus_RAF --KFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHE---GL-TVKIGDFGLATV KSR1_Spur_KSR --RFNINRTIQIATQITQGMSYLHHKGIVHKDLKSKNIFIEN----G-KVVLTDFALFSI Raf_Spur_RAF --KLVMHQLIDISRQTAQGMDYLHAKNIIHRDLKSNNIFLHD---DL-TVKIGDFGLATV KSR2_Spur_KSR --KLQTSSKMDIARQIGQAMGYLQAKGIIVGHLSSRCVYLES---KV-KLSVTRVDTSDV AqueK675_Aque_KSR --NFTTPSKLKILRQISEGMEYLHAKGIQHGGLSSKLVTINH---R-VCISLS-F----- AqueK676_Aque_KSR --AFSLERLANIGRQIAQAMGYLHARGIIHKGLNTKNIFLEKRDQKE-KVIITDMGLSSL AqueK677_Aque_RAF --RFEMYKIIDICRQTAQGVDYLHAKQIIHRDLKSNNIFLHD---DM-TVKIGDFGLATV AqueK678_Aque_RAF --RFEMYKIIDICRQTAQGVDYLHAKQIIHRDLKSNNIFLHD---DM-TVKIGDFGLATV AqueK679_Aque_RAF --PLPFHQEINISLDVARALDYLHVNNIHHRDLSAKNILMLG---DY-RAKVSDFGQSKL : : : .: **: . * : :. : : . RAF1_Hsap_RAF KSRW---SG--SQQVEQPTGSVLWMAPEVIRMQ-----------DNNP--FSFQSDVYSY KSR2_Hsap_KSR SGVLQAGR--REDKLRIQNGWLCHLAPEIIRQLSPDT-----EEDKLP--FSKHSDVFAL KSR1_Hsap_KSR SGVVREGR--RENQLKLSHDWLCYLAPEIVREMTPGK-----DEDQLP--FSKAADVYAF phl_Dmel_RAF KTRW---SG--EKQANQPTGSILWMAPEVIRMQ-----------ELNP--YSFQSDVYAF ksr_Dmel_KSR TKLLYC-----DMGLGVPHNWLCYLAPELIRALQPEK----PRGECLE--FTPYSDVYSF KSR2_Mmus_KSR SGVLQAGR--RDDKLRIQNGWLCHLAPEIIRQLSPDT-----EEDKLP--FSKHSDVFAL KSR1_Mmus_KSR SGVVREER--RENQLKLSHDWLCYLAPEIVREMIPGR-----DEDQLP--FSKAADVYAF BRAF_Mmus_RAF KSRW---SG--SHQFEQLSGSILWMAPEVIRMQ-----------DKNP--YSFQSDVYAF ARAF_Mmus_RAF KTRW---SG--AQPLEQPSGSVLWMAAEVIRMQ-----------DPNP--YSFQSDVYAY lin-45_Cele_RAF KTKWTV-NG--GQQQQQPTGSILWMAPEVIRMQ-----------DDNP--YTPQSDVYSF ksr-2_Cele_RAF ERLENP-R--RNCTLLIPNHWIDYLSPEIAGNLMIDWRGDVLFQHELP--FSQESDVYSF ksr-1_Cele_KSR KRLAHP-K--QKSGYLTSKFWTNYIAPELAMAMRTEYDE--YECDDFP--FSENSDVYAF BRAF_Hsap_RAF KSRW---SG--SHQFEQLSGSILWMAPEVIRMQ-----------DKNP--YSFQSDVYAF ARAF_Hsap_RAF KTRW---SG--AQPLEQPSGSVLWMAAEVIRMQ-----------DPNP--YSFQSDVYAY RAF1_Mmus_RAF KSRW---SG--SQQVEQPTGSVLWMAPEVIRMQ-----------DDNP--FSFQSDVYSY KSR1_Spur_KSR GRLSKQKT--RKDSLVIPDGWLCYLAPELITSLKINTDG--TQDMELP--FSEKSDVYSF Raf_Spur_RAF KSRW---SG--SQQFEQPSGSILWMAPEVIRMR-----------DTNP--YSFQSDVYAF KSR2_Spur_KSR T-----CQRA--SHACLPRGKLQYLAPELLAKARVVPP---EFITGNE--QTHSTDVYAF AqueK675_Aque_KSR -----HN--TNNR--QLEGCDLPYLTPEEIRSITQLSNG--YLFLP--TKPTPSTDIFAF AqueK676_Aque_KSR SSLLYDNTTSKGGVVTFPRAQLYSLAPEIIRKISPTPV---VQIDQEPYSYNYATDVYAF AqueK677_Aque_RAF KTRW---DG--NEKVRQPTGSILWMAPEVIRME-----------EPDP--YTFYCDVYSF AqueK678_Aque_RAF KTRW---DG--NEKVRQPTGSILWMAPEVIRME-----------EPDP--YTFYCDVYSF AqueK679_Aque_RAF RNPNG---G--FSSNSPCPGNILYMPPETLKV-------------PPD--FAPTLDEFSF :..* * :: RAF1_Hsap_RAF GIVLYELMTGELPYSHIN-----NRDQIIFMVGRGYA-SPDLSK----L-YKNCPKAMKR KSR2_Hsap_KSR GTIWYELHAREWPFKTQP------AEAIIWQMGTG--MKPNLSQ-------IGMGKEISD KSR1_Hsap_KSR GTVWYELQARDWPLKNQA------AEASIWQIGSGEGMKRVLTS-------VSLGKEVSE phl_Dmel_RAF GIVMYELLAECLPYGHIS-----NKDQILFMVGRGLL-RPDMSQ----V-RSDAPQALKR ksr_Dmel_KSR GTVWYELICGEFTFKDQP------AESIIWQVGRG--MKQSLAN-------LQSGRDVKD KSR2_Mmus_KSR GTIWYELHAREWPFKTQP------AEAIIWQMGTG--MKPNLSQ-------IGMGKEISD KSR1_Mmus_KSR GTVWYELQARDWPFKHQP------AEALIWQIGSGEGVRRVLAS-------VSLGKEVGE BRAF_Mmus_RAF GIVLYELMTGQLPYSNIN-----NRDQIIFMVGRGYL-SPDLSK----V-RSNCPKAMKR ARAF_Mmus_RAF GVVLYELMTGSLPYSHIG-----SRDQIIFMVGRGYL-SPDLSK----I-FSNCPKAMRR lin-45_Cele_RAF GICMYEILSSHLPYSNIN-----NRDQILFMVGRGYL-RPDRSK----I-RHDTPKSMLK ksr-2_Cele_RAF GTIFFELLLRRMPTGCDS------WDQKLYAKMCG--QKAALQRLDA-Q-LQKIDGKLHE ksr-1_Cele_KSR GCVWFEMLTGALPYAGEL------PHQILFAKTQG--IRPVLPN-------VKCTQELKE BRAF_Hsap_RAF GIVLYELMTGQLPYSNIN-----NRDQIIFMVGRGYL-SPDLSK----V-RSNCPKAMKR ARAF_Hsap_RAF GVVLYELMTGSLPYSHIG-----CRDQIIFMVGRGYL-SPDLSK----I-SSNCPKAMRR RAF1_Mmus_RAF GIVLYELMAGELPYAHIN-----NRDQIIFMVGRGYA-SPDLSR----L-YKNCPKAMKR KSR1_Spur_KSR GTIWYELLAGEWPFHKQP------VETVIWQVGKG--LKQPLNH-------LQASRDVKE Raf_Spur_RAF GVVLFELVTQSLPYQNIK-----HKDQIIWMVGRGYL-QPDLSK----V-RNDTPKALKR KSR2_Spur_KSR GTILFEIIAGRWPLQYAC------SESVIYQTCSG--RRDDFRN-------VTCSSTMKN AqueK675_Aque_KSR GTIAYELTTLKLPFLNQS------LLDLIWSVGNNG-HQ-SLK---L-L-P---RSCFRS AqueK676_Aque_KSR GTILYELITKRFPFSTDSLGFAYEFEVVIFLVGHG--HRQPFKG--------ETPKKLKD AqueK677_Aque_RAF GVVVYELISGQLPYPHVQ-----DRDQILFMVGRGFL-RPDPSS----A-RKDTPKSFKQ AqueK678_Aque_RAF GVVVYELISGQLPYPHVQ-----DRDQILFMVGRGFL-RPDPSS----A-RKDTPKSFKQ AqueK679_Aque_RAF GIVTIQIMSRKDPKPSDL-----HRTG--SLIGTLVE-VPEIERRRDDIDSCDPSNPILG * :: . . RAF1_Hsap_RAF LVADCVKKVKEERPLFPQILSSI-----------------------EL KSR2_Hsap_KSR ILLFCWAFEQEERPTFTKLM--------------------------DM KSR1_Hsap_KSR ILSACWAFDLQERPSFSLLM--------------------------DM phl_Dmel_RAF LAEDCIKYTPKDRPLFRPLL--------------------------NM ksr_Dmel_KSR LLMLCWTYEKEHRPQFARLLS-------------------------LL KSR2_Mmus_KSR ILLFCWAFEQEERPTFTKLM--------------------------DM KSR1_Mmus_KSR ILSACWAFDLQERPSFSLLM--------------------------DM BRAF_Mmus_RAF LMAECLKKKRDERPLFPQILASI-----------------------EL ARAF_Mmus_RAF LLTDCLKFQREERPLFPQILA-------------------------TI lin-45_Cele_RAF LYDNCIMFDRNERPVFGEVLE-------------------------RL ksr-2_Cele_RAF LLLECWSSQPEKRPSFQQIVKRITV------------------QMPRK ksr-1_Cele_KSR LLVSCWNTAPQDRPTLTDINLKLTALP----------KKPRVNRSPSF BRAF_Hsap_RAF LMAECLKKKRDERPLFPQILASI-----------------------EL ARAF_Hsap_RAF LLSDCLKFQREERPLFPQILA-------------------------TI RAF1_Mmus_RAF LVADCVKKVKEERPLFPQILSSI-----------------------EL KSR1_Spur_KSR ILMMCWSYSPDSRPIFQHIKK-------------------------TI Raf_Spur_RAF LITDCYALNRDERPLFPVVLA-------------------------AL KSR2_Spur_KSR LIQECWSQNPNERPSFQDIVKELKKNVSL-----------GNMKHSLS AqueK675_Aque_KSR VIHRCWSH--TRRTTFKDILSSI-----------------------QD AqueK676_Aque_KSR IVWECWSHQPQDRPPFSDLQERLARLCRKHTPITRSPSHPINLSKSVD AqueK677_Aque_RAF LCIKCCQFEKDDRPLFHQILADL-----------------------ES AqueK678_Aque_RAF LCIKCCQFEKDDRPLFHQVYNNL----------------------ESL AqueK679_Aque_RAF LSISCISNDPQERPPIERMCQLL----------------------SLL : * *. : :