CLUSTAL W (1.83) multiple sequence alignment Gprk1_Dmel MADLEAVLADVSYLMAMEKSKCTPAARASKKLNLPDPSVRSVMYKYLEKEGELNFHKNFN BARK2_Mmus MADLEAVLADVSYLMAMEKSKTAPAARASKKVVLPEPSIRSVMQRYLAERNEITFDKIFN BARK1_Mmus MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFS BARK1_Hsap MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFS BARK2_Hsap MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFN W02B3.2_Cele MADLEAVLADVSYLMAMEKSRSQPAARASKRIVLPDPSVRSIMQKFLEKSGDMKFDKIFN BARK_Spur MADLEAVLADVSYLMAMEKSKSTPAARASKKLVLPDPSVRTVMYKYLEERKEITFEKIFG AqueK007_Aque MADLESVLADVSYLMTIEKSR-NPGAKTLKKIVLPDNSVHDVVHKWLSFKNELNFHRIHK *****:*********::***: *.*:: *:: **: *:: :: ::* ::.*.: . Gprk1_Dmel EVLGYLLFKDFCENDSEEPIQQLKFFEQIKLFEKTECYDERKKMARDIYDNFIMEEMLSH BARK2_Mmus QKIGFLLFKDFCLNEIGEAVPQVKFYEEIKEYEKLDNEEDRLRRSRQMYDAYIMRELLSS BARK1_Mmus QKLGYLLFRDFCLNHLEEAKPLVEFYEEIKKYEKLETEEERVVRSREIFDSYIMKELLAC BARK1_Hsap QKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLAC BARK2_Hsap QKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSC W02B3.2_Cele QKLGFLLLKDYAENVSESPCPQIKFYEAIKEYEKMETPDERLTKAREIYDHHIMVEMLAH BARK_Spur QKLGYLLFKDYCENCADVQVQQLQFYEAIKDYEKLDTLDERLEEARRIFDNYIMKEVLSC AqueK007_Aque QRMGTYFLEKFIP----PLCVTYSFLKAILDYEKTSSIVSRQKKAKQIYDKYIYADMLAM : :* ::..: .* : * :** . .* :: ::* .* ::*: Gprk1_Dmel TYEYSKHAVASVQKYLLKNEVPVDLFEPYLEEIFTQLKGKPFKKFLESDKFTRFCQWKNL BARK2_Mmus THQFSKQAVEHVQSHLSKKQVTATLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNV BARK1_Mmus SHPFSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNV BARK1_Hsap SHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNV BARK2_Hsap SHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNV W02B3.2_Cele AHNYSKESLQHVQYHLLKQNVPPDLFHRYVLEICDQLRGDIFQRFLESDKFTRFCQWKNL BARK_Spur THQFSKSAVENVRQRLTNQEAKPDLFSDYITEILNSLKGEIFQKFLESDKFTRFCQWKNV AqueK007_Aque SSKIPKEITDKVTVSLKANEVSTKLYGEIKEYLYDELTGLYMKDFIHSSHYVRYCQWKYI : .* * * ::. *: : .* * :: *:.*.::.*:**** : Gprk1_Dmel ELNIQ--LTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGEMLALN BARK2_Mmus ELNIH--LSMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRVKMKQGETLALN BARK1_Mmus ELNIH--LTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALN BARK1_Hsap ELNIH--LTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALN BARK2_Hsap ELNIH--LTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALN W02B3.2_Cele ELNMQ--LTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALN BARK_Spur ELNINGMLSMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKSGETLALN AqueK007_Aque ELSVPHTISLSDFNVHRIIGRGGFGEVFGCRKDDSGKMFAMKLLDKKRIKVKRGELLALN **.: :::.:*.*************:**** *:***:*** *****:*:* ** **** Gprk1_Dmel ERNMLQAVSTGIDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGIFSEDEMKFY BARK2_Mmus ERIMLSLVSTG-DCPFIVCMTYAFHTPDKLCFILDLMNGGDMHYHLSQHGVFSEKEMRFY BARK1_Mmus ERIMLSLVSTG-DCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFY BARK1_Hsap ERIMLSLVSTG-DCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFY BARK2_Hsap ERIMLSLVSTG-DCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFY W02B3.2_Cele ERIMLSLVSTGQDCPFIVCMTYAFQSPDKLCFILDLMNGGDLHYHLSQHGVFTEQEMIFY BARK_Spur ERIMLSLVSET-DCPFIVCMTYAFQTPDKLCFILDLMNGGDLHYHLSQHGVFSEEEVGFY AqueK007_Aque ERNMLAKVN----SPFVVNLYYAFQTPEKLCFILDLMNGGDLHYHLTQHGVFNEAEVRFY ** ** *. .**:* : ***::*:**.**********:****:***:*.* :: ** Gprk1_Dmel AAEVILGLEHMHKRCIVYRDLKPANILLDENGHIRISDLGLACDFSKKKPHASVGTHGYM BARK2_Mmus ASEIILGLEHMHTCFVVYRDLKPANILLDEYGHVRISDLGLACDFSKKKPHASVGTHGYM BARK1_Mmus AAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKRPHASVGTHGYM BARK1_Hsap AAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYM BARK2_Hsap ATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYM W02B3.2_Cele ASEVILGLEHMHNRFVVYRDLKPANILLDENGHVRVSDLGLACDYSKKKPHASVGTHGYM BARK_Spur AAEIILGLEHMHVRNVVYRDLKPANILLDENGHVRISDLGLACDFSSKKPHASVGTHGYM AqueK007_Aque AAELILALEHIHSQLIVYRDLKPSNVLLDEAGHIRLSDLGLACEFSDRKPSSCVGTHGYM *:*:**.***:* :*******:*:**** ** *:*******::*.::* :.******* Gprk1_Dmel APEVLSKGTSYD------------------------------------SCADWFSFGCML BARK2_Mmus APEVLQKGTCYD------------------------------------SSADWFSLGCML BARK1_Mmus APEVLQKGVAYD------------------------------------SSADWFSLGCML BARK1_Hsap APEVLQKGVAYD------------------------------------SSADWFSLGCML BARK2_Hsap APEVLQKGTAYD------------------------------------SSADWFSLGCML W02B3.2_Cele APEVLAKGVAYD------------------------------------SSADWFSLGCML BARK_Spur APEVLSKGTAYDSSADWFSLGCMLFKLLHGGTHGYMAPEVLSKGTAYDSSADWFSLGCML AqueK007_Aque APEVIQKGVHYD------------------------------------KSADWFSLGCVL ****: **. ** ..*****:**:* Gprk1_Dmel YKLLKGHSPFRQHKTKDKLEIDKMTLTMNVELPE-SFSLELKNLLEMLLQRDVSKRLGCM BARK2_Mmus FKLLRGHSPFRQHKTKDKHEIDRMTLTVNVQLPD-AFSPELRSLLEGLLQRDVSQRLGCG BARK1_Mmus FKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPD-SFSPELRSLLEGLLQRDVNRRLGCL BARK1_Hsap FKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPD-SFSPELRSLLEGLLQRDVNRRLGCL BARK2_Hsap FKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPD-TFSPELKSLLEGLLQRDVSKRLGCH W02B3.2_Cele YKLLKGHSPFRQHKSKDKNEIDKMTLTQDIELPNEGLSKDCRDLLEGLLKRDVPDRLGCR BARK_Spur FKLLHGHSPFRQHKTKDKHEIDRMTLTMDVEFPD-KMSDEMRALLAGLLQREVASRLGCE AqueK007_Aque YKLLRGHSPFRS---QNKDEVDQITLTKEVTMPS-SFSPGVSQLLTGLLEKDPTKRLGCL :***:******. ::* *:*::*** : :*. :* ** **::: **** Gprk1_Dmel GNGADEVKMHNFFCGIDWHQVYIQKYTPPLVPPRGEVNAADAFDIGSFDEEDTKGIKLND BARK2_Mmus GGGARELKEHIFFKGIDWQHVYLRKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLD BARK1_Mmus GRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLD BARK1_Hsap GRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLD BARK2_Hsap GGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLD W02B3.2_Cele GKGPTEVKEHPFFKDVDWQTVYLRRMTPPLIPPRGEVNAADAFDIGNFDDDEVKGVKLQD BARK_Spur GRGATEVREHPFFKTTDWNQVYYQKVQPPLIPPRGEVNAADAFDIGSFDEDDVKGIKVRN AqueK007_Aque DTGVDAIKELPFFSDLNWDDVMEKKYAPPLIPPRGEVNAADAFDIGSFDSDETRGVKLND . * :: ** :*. * :: ***:***************.**.::.:*:*: : Gprk1_Dmel ADQDLYKMFSLTISERWQQEVSETVFDTVNTETDKLEQKRKLKQKQ--H-FDADE--KES BARK2_Mmus CDQDLYKNFPLVISERWQQEVVETIYDAVNADTDKIEARRKAKNKQ--L-GQEEDYAMGK BARK1_Mmus SDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQ--L-GHEEDYALGK BARK1_Hsap SDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQ--L-GHEEDYALGK BARK2_Hsap CDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQ--L-GHEEDYALGK W02B3.2_Cele GDSDLYKNFNIVISERWQNEIAETIFEVVNQDADKAESKKRSKQKI--K-VAVEE--KDS BARK_Spur L----------------------------------------------------------- AqueK007_Aque EDQKMYSRFNVVVSDRWQAEMMESVFDSVNQDKEKQEGKERSKSKNGILPFYTADGSFTD Gprk1_Dmel DCILHGYIKKLGG-SFASLWQTKYAKLYPNRLELHSESGNNKPELIFM---DQVEDISSD BARK2_Mmus DCIMHGYMLKLGN-PFLTQWQRRYFYLFPNRLEWRGEG-ESRQSLLTM---EQIMSV--E BARK1_Mmus DCIVHGYMSKMGN-PFLTQWQRRYFYLFPNRLEWRGEG-EAPQSLLTM---EEIQSV--E BARK1_Hsap DCIMHGYMSKMGN-PFLTQWQRRYFYLFPNRLEWRGEG-EAPQSLLTM---EEIQSV--E BARK2_Hsap DCIMHGYMLKLGN-PFLTQWQRRYFYLFPNRLEWRGEG-ESRQNLLTM---EQILSV--E W02B3.2_Cele DVIVHGYIKKLGG-PFTSAWQTKYGKLYPSRLELYPESLTAKPELVFM---DQIEDVCAE BARK_Spur --------------PFLTFL---------------------------------------- AqueK007_Aque DSLLQGYGSKQSTAAMFTHWQRKYFKLYPNRLDIGDSFTSMSKGLVSISFTESSILV--E .: : Gprk1_Dmel FILHKNENCIQIRINDGTRDGRIILTNS-----------------------DEIGLKEWS BARK2_Mmus ETQIKDRKCILLRIKGGKQ---FVLQCE-----------------------SDPEFAQWL BARK1_Mmus ETQIKERKCLLLKIRGGKQ---FVLQCD-----------------------SDPELVQWK BARK1_Hsap ETQIKERKCLLLKIRGGKQ---FILQCD-----------------------SDPELVQWK BARK2_Hsap ETQIKDKKCILFRIKGGKQ---FVLQCE-----------------------SDPEFVQWK W02B3.2_Cele MQTIKGETAIIVKLRDGFK---EPKICLTNSVSENVLKYKTEKKNCRSVYLDEISLKEWH BARK_Spur ------------------------------------------------------------ AqueK007_Aque SKQIKGTDIIRLQPIGTRR--ETCIKFE-----------------------SQLEHQMWF Gprk1_Dmel SSLRSAHKISQDLLGSMAKKAGKIY----GSERDVNKSM-YIFG------------GNCS BARK2_Mmus KELTCTFNEAQRLLRRAPKFLNKPR----AAILEFSKPP------------------LCH BARK1_Mmus KELRDAYREAQQLVQRVPKMKNKPR----SPVVELSKVP------------------LIQ BARK1_Hsap KELRDAYREAQQLVQRVPKMKNKPR----SPVVELSKVP------------------LVQ BARK2_Hsap KELNETFKEAQRLLRRAPKFLNKPR----SGTVELPKPS------------------LCH W02B3.2_Cele TSLRTAHKVSQELLQRMGRKAIKIYGVNHDPMLSESERPGSVTR------------AFLN BARK_Spur ------------------------------------------------------------ AqueK007_Aque RFLRDAIHKHDST---------KDP----SGPIALEDGG-LIQRRQISDSPARRARELGY Gprk1_Dmel TKTS-NGSN BARK2_Mmus R-NS--SGL BARK1_Mmus R-GS-ANGL BARK1_Hsap R-GS-ANGL BARK2_Hsap R-NS--NGL W02B3.2_Cele RASSVDSGV BARK_Spur -----NLKT AqueK007_Aque TR-S-VPIL