CLUSTAL W (1.83) multiple sequence alignment PAK6_Hsap -------------------------MFRK-------------KKKKRPEISAPQNFQHRV PAK5_Hsap -------------------------MFGK-------------K-KKKIEISGPSNFEHRV PAK4_Hsap -------------------------MFGK-------------R-KKRVEISAPSNFEHRV PAK6_Mmus -------------------------MFRK-------------KKKKRPEISAPQNFQHRV PAK5_Mmus -------------------------MFGK-------------K-KKKIEISGPSNFEHRV PAK4_Mmus -------------------------MFGK-------------K-KKRVEISAPSNFEHRV C45B11.1_Cele MKISINISKQSLTISYAEKKMNRTFSLRR--------------KVKKSEISTPSNFEHRI mbt_Dmel -------------------------MFSK-------------K-KKKPLISMPSNFEHRV PAK4_Spur MGNA-----------------LK--MTSK-------------KRNGKVKISTPSNFEHRV AqueK236_Aque MP-----------------------LFSRSRSLRRSSKRRSVHRIKKEDISEPMGFMHCY : : ** * .* * PAK6_Hsap HTSFDPKEGKFVGLPPQWQNIL----------------------------------DTLR PAK5_Hsap HTGFDPQEQKFTGLPQQWHSLLA---------------------------------DTAN PAK4_Hsap HTGFDQHEQKFTGLPRQWQSLIE---------------------------------ESAR PAK6_Mmus HTSFDPKEGKFVGLPPQWQNIL----------------------------------DTLR PAK5_Mmus HTGFDPQEQKFTGLPQQWHSLLA---------------------------------DTAN PAK4_Mmus HTGFDQHEQKFTGLPRQWQSLIE---------------------------------ESAR C45B11.1_Cele HAGFDARSGTYTGLPKQWQALLGPP-------------------------------RSIS mbt_Dmel HTGFDKRENKYVGLPLQWASIVGNNQI----------------------------LKSSN PAK4_Spur HTGYDRRLGKYVGLPPQWASIIG---------------------------------AEET AqueK236_Aque HAEIG--SEGFSGLPPQWKTLVDTTHPSTTSPTTSRATSASPSNKNETSTKRIGSKSTGN *: . : *** ** :: PAK6_Hsap RPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYI---SG--------------------- PAK5_Hsap RPKPMVDPSCITPIQLAPMKTIVRGNKPCKETSI---NG--------------------- PAK4_Hsap RPKPLVDPACITSIQPGAPKTIVRGSKGAKDGAL---TL--------------------- PAK6_Mmus RPKPVVDPSRITRVQLQPMKTVVRGSSVPTEGYI---SG--------------------- PAK5_Mmus RPKPMVDPSCITPIQLAPMKTIVRGNKSCKETSI---NG--------------------- PAK4_Mmus RPKPLIDPACITSIQPGAPKTIVRGSKGAKDGAL---TL--------------------- C45B11.1_Cele RPKPMVDPSCITPVDVAELKTVIRGPSSSFRYN-SPLPF--------------------- mbt_Dmel RPLPLVDPSEITPTEILDLKTIVRPHHNNNKADTTSLNSSSTMMMGSMAPMNPMAPGAHP PAK4_Spur RPKPIVDPSCITQTEAFELKGIVRGSNAQSPE-Q---NG--------------------- AqueK236_Aque TPEPIKRPSP-----------IVRGSDSCLEDTV---KY--------------------- * *: *: ::* PAK6_Hsap ---------------LLNDIQKLSV--------ISSNTLRGRS--PTSRRRAQS-LGL-- PAK5_Hsap ---------------LLEDFDNISV--------TRSNSLRKES--PPTPDQGASSHGPGH PAK4_Hsap ---------------LLDEFENMSV--------TRSNSLRRDS--PPPPARARQENGM-- PAK6_Mmus ---------------LLNDIQKLSV--------ISSNTLRGRS--PTSRRRAQS-LGL-- PAK5_Mmus ---------------LLEDFDNISV--------TRSNSLRKES--PPTPDQGAASRIQGH PAK4_Mmus ---------------LLDEFENMSV--------TRSNSLRRES--PPPPARAHQENGM-- C45B11.1_Cele ---------------GMTNSPMPSV--------ARSNSLRISA--TASPVVNVSSA---- mbt_Dmel MMSHGPGMMMPPETGGIVLPKTSHV--------ARSNSLRSSS--PPRVRRVANVPPS-V PAK4_Spur ------------------TKPRMSV--------ARSNSLRSRG-SPPRSRRRNQEEDP-Q AqueK236_Aque ---------------VHDHCCSLAGEEDEEVHEFIDIQLRSQTGGSSTGSQGSSSRH--- . ** .. PAK6_Hsap LGD--------------------EHWATDPD-----------------------MYLQSP PAK5_Hsap AEENG--------------FITFSQYSSESDTTADYTT--EKYREKSLYGDDLDPYYRGS PAK4_Hsap PEEPA--------------------------TT-----------------ARGGPGKAGS PAK6_Mmus LGD--------------------DQWAADPD-----------------------MYLQSP PAK5_Mmus SEENG--------------FITFSQYSSESDTTADYTT--EKYRDRSLYGDDLDLYYKSS PAK4_Mmus LEERA--------------------------AP-----------------ARMAPDKAGS C45B11.1_Cele ------------------------------------------------------------ mbt_Dmel PEEEGPPAAGTPG---------------------------------------VGGASSGG PAK4_Spur -GQQGPQGGG-EKKPEDNTKIPAHPYDQNPNSSAQQMMMNREMRD------RLNSKGSGG AqueK236_Aque SSQQQ------------LASPPFSTVAVTTPTIP-----PLSTRSSTL-----------S PAK6_Hsap QSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQ-SLG---P------- PAK5_Hsap HAAKQNGHVMKMK--------HGEAYYSEVKPLKSDFARFSA----DYHSHLDSLSKPSE PAK4_Hsap R-GRFAGHS--------------------------------------------------- PAK6_Mmus QSEHTDPHGLYLSCNGGTPAGHRQVPWPEPQSPQALPNGMAAKAQ-SLG---P------- PAK5_Mmus HAAKQNGHAMKMK--------HGDAYYPEMKSLKTDLAGFPV----DYHTHLDSLRKSSE PAK4_Mmus R-ARATGHS--------------------------------------------------- C45B11.1_Cele ------------------------------------------------------------ mbt_Dmel F-KPPGAHP------------------------SLLYNSQHAHANGATG---PLAVRTDQ PAK4_Spur S-GPPPNHP------------------------DQR--GGQSWSRGGSHEKRSATTRNDP AqueK236_Aque HIS--SDH-----------------SHHHPSSPFSFTAPLDAIQS-DLG---L------- PAK6_Hsap -----AEFQGASQRCL-Q---LGACLQSSPPGASPPTGTNRHGMKA------AKHGSEEA PAK5_Hsap YSDLKWEYQRASSSS--P---LDYSFQFTPSRTAGTSGCSKESLAYSESEWGPSLDDYDR PAK4_Hsap -------------------------------EAGGGSGD--------RR-----RAGPEK PAK6_Mmus -----AEFQGASQRCLQQ---LGACLQSSPPGTSPPMATGRRGVKV------AKHSSEEA PAK5_Mmus YGDLRWDYQRASSSS--P---LDYSFQLTPSRTAGTSRCSKESLAYSESDWGPSLDDYDR PAK4_Mmus -------------------------------EAGSGSGD--------RR-----RVGPEK C45B11.1_Cele -----------------------------------------------RH-----SFRPTL mbt_Dmel TN-L----QQ--------------------------------------------YRSNLA PAK4_Spur RNDPRNDPRQNPRQD--PRQDPRYDPRNDPRRTDPRNQDPRNRDPRNQDPRNQDPRYQDR AqueK236_Aque -----YDSEGSSSV-LT----TSSHIIYSPSDSSGYFGSTHSSLYS------SRLSTSQH PAK6_Hsap RPQSCLVGSATGRPGGEGSPSPKTRESSLKRR--LFRSMFLSTAATAPPSSSKPGPPPQS PAK5_Hsap RPKSSYLN--------QTSPQPTMRQ-----R--SRSGSGLQEPMM-PFGASAFKTHPQG PAK4_Hsap RPKSSREG--------SGGPQESSRD----KR--PLSGPDVGTPQ--PAGLASGAKLAAG PAK6_Mmus RPQSCLVGSAIGRPGGEGSPSPKNQESSLKHR--LFRSMFLSTPATGAASSSKPVPLPQN PAK5_Mmus RPKSSYLH--------QTSPQPAMRQ-----R--SKSGSGLQEPMM-PFGASAFKTHPQG PAK4_Mmus RPKSSRDG--------PGGPQEASRD----KR--PLSGPDVSTPQ--PGSLTSGTKLAAG C45B11.1_Cele PPVSQ---------------------R--------------------------------G mbt_Dmel PPS----G--------GSMPQQQ---------QTSPVG--SVASGT-RS----------- PAK4_Spur RPD----G--------HSLPRGASGD----PRAHPPQG--QGPPGY-PGGPGGPRGDPRG AqueK236_Aque IPSSAL-----PPP-PPVAPLPSTSQYSTH----------QSPP--------------HH * PAK6_Hsap KPNSSFR---------------------------------------------PPQKDNP- PAK5_Hsap HSYNSYTYPRLSEPTMCIPKVDYDRAQM--VLSPPLSGSDTYPRGPA----KLPQSQS-- PAK4_Hsap RPFNTYPRADTDHPSRG-------------AQGEPH---DVAPNGPSAGGLAIPQSSS-- PAK6_Mmus KPNSAFR---------------------------------------------PPQKDSS- PAK5_Mmus HSYNSYTYPRLSEPTMCIPKVDYDRAQM--VFSPPLSGSDTYPRGPT----KLPQSQS-- PAK4_Mmus RPFNTYPRADTDHPPRG-------------AQGEPH---TMAPNGPSATGLAAPQSS--- C45B11.1_Cele YPF----------------------------------------------------NDPSY mbt_Dmel ------------NH-----------------------SH---TNNGNSGGSYPPMYPTSH PAK4_Spur DP-RGY---QRDSTSSG-------------GSGPRTTGS---PGQPQPPGGFPPRHPDAQ AqueK236_Aque RPLGP--------IHSCLP---FDTTSTDASNALPS--SSTH----DVAGVQYMHHYHNH PAK6_Hsap PSLVAK-----------AQ---------SLPSDQ----PVGTFSPLTTSDTSSPQKSLRT PAK5_Hsap ----------KSGYSSSSH---------QYPSGYHKATLYHH-PSLQSSSQYISTASYLS PAK4_Hsap ----------------SSS---------RPPTRARGAPSPGVLGPHASEPQLAPPACTPA PAK6_Mmus SNLVAK-----------AQ---------SLPSEQ----PMGTFSPLTTSDTSSPQKSLRT PAK5_Mmus ----------KAGYSSGSH---------QYPSGYHKASLYHH-PSLQTSSQYISTASYLS PAK4_Mmus ----------------SSS---------RPPTRARGAPSPGVLGPHASEPQLAPPARALA C45B11.1_Cele APLPLR-----------NQ---------KPPMST----TFGVEKPHQYQ-QIITIV-APS mbt_Dmel QQQQQQQQ--QAKQGGDQN---------QNPLHPHAHPHPHHHQHLAKSASRASSSSGGA PAK4_Spur GRHPLQ-----------NR---------QPPPQGYGGPPPPGQQQQ-----QRPPGQMPH AqueK236_Aque PHFSSLQRPAKSGTSPSRNTQRIRRSDLMNPSHATATPVS-H-VMTTSSSRNITSST-GA * . PAK6_Hsap APATGQLPGRSSPAGSPRTWHAQISTSNLYLPQ---DPTVAKGA---LAGEDTGVVTHEQ PAK5_Hsap SLSL----------------------S---SST---YPPPSWGS---SSDQQPSRVSHEQ PAK4_Hsap APAV---------------------------------PGPPGPR---SPQREPQRVSHEQ PAK6_Mmus APAAGPLPGRSSPAGSPRTRHAQISTSNLYLPQ---DPTVAKGA---LGGEDTGIVTHEQ PAK5_Mmus SLSI----------------------S---SST---YPPPSWGS---SSDQQPSRVSHEQ PAK4_Mmus APAV---------------------------PP---APGPPGPR---SPQREPQRVSHEQ C45B11.1_Cele R-T----------------------TTPQ-LQP----KSPSTPQAMRQQPKCTEGVSDEE mbt_Dmel SSA---------------------------AQQ---ASGASGGA--AGQPKQDQRLTHEQ PAK4_Spur ------------------------------SNS---SPSPSNP---T-PNPGEQHVSHEQ AqueK236_Aque AVN---------P----H----F--VSDV-YSTGRGHTVPAKP---H-RNRPSSKMSAEQ . :: *: PAK6_Hsap FKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELL PAK5_Hsap FRAALQLVVSPGDPREYLANFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELL PAK4_Hsap FRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELL PAK6_Mmus FKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELL PAK5_Mmus FRAALQLVVSPGDPREYLDNFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRRELL PAK4_Mmus FRAALQLVVDPGDPRSYLDNFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELL C45B11.1_Cele FRNALKFVVDGTDPRSDLTDYKQIGEGSTGVVEAAYKISTKQIVAVKRMNLRKQQRRELL mbt_Dmel FRAALQMVVSAGDPRENLDHFNKIGEGSTGTVCIATDKSTGRQVAVKKMDLRKQQRRELL PAK4_Spur FRSALQMVVSPGDPRYKLENFVKIGEGSTGIVCIATEASSGRQVAVKKMDLRKQQRRELL AqueK236_Aque FRATISLLVNSSDPRRDLDWFVKIGEGSTGTVYTAHQLSTNRVVAVKKMSLWNQQRKELL *: :: ::*. *** * : :******* * * : : **** *.* :***:*** PAK6_Hsap FNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQV-RLNEEQIATVCE PAK5_Hsap FNEVVIMRDYHHDNVVDMYSSYLVGDELWVVMEFLEGGALTDIVTHT-RMNEEQIATVCL PAK4_Hsap FNEVVIMRDYQHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHT-RMNEEQIAAVCL PAK6_Mmus FNEVVIMRDYQHLNVVEMYKSYLVGEELWVLMEFLQGGALTDIISQV-RLNEEQIATVCE PAK5_Mmus FNEVVIMRDYHHDNVVDMYNSYLVGDELWVVMEFLEGGALTDIVTHT-RMNEEQIATVCL PAK4_Mmus FNEVVIMRDYRHENVVEMYNSYLVGDELWVVMEFLEGGALTDIVTHT-RMNEEQIAAVCL C45B11.1_Cele FNEVSILRQYQHPNIVRFFSSHLVDDELWVVMEFMEGGSLTDIVTAT-RMTEPQIATISR mbt_Dmel FNEVVIMRDYHHPNIVETYSSFLVNDELWVVMEYLEGGALTDIVTHS-RMDEEQIATVCK PAK4_Spur FNEVVIMRDYKHPNIVEMYDSFLVGDELWVVMEFLEGGALTDIVTHTGRMQEEQVAYVCQ AqueK236_Aque FNEVIIMKEYPHNNVVTYYDSYLVDEELWVLMEFIDGGSLTDIIRRV-HLQESQIAAICK **** *:::* * *:* :.*.**.:****:**:::**:****: :: * *:* :. PAK6_Hsap AVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWM PAK5_Hsap SVLRALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWM PAK4_Hsap AVLQALSVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWM PAK6_Mmus AVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWM PAK5_Mmus SVLKALSYLHNQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWM PAK4_Mmus AVLQALAVLHAQGVIHRDIKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWM C45B11.1_Cele QVLGALDFLHARKVIHRDIKSDSILLKRDGTVKLTDFGFCGQLSEEVPRRRSLVGTPYWT mbt_Dmel QCLKALAYLHSQGVIHRDIKSDSILLAADGRVKLSDFGFCAQVSQELPKRKSLVGTPYWM PAK4_Spur AILKALAFLHSQGVIHRDIKSDSILLTHDGNVKLSDFGFCAQISSDTPKRKSLVGTPYWM AqueK236_Aque SCLEALAYLHSNGVIHRDIKSDCILLMSDGTVKLSDFGYCARINQKQRRRQSLVGTPYWM * ** ** . *********.*** ** :**:***:*.::... :*:******** PAK6_Hsap APEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVS PAK5_Hsap APEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVS PAK4_Hsap APELISRLPYGPEVDIWSLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVS PAK6_Mmus APEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSAPPKLKNSYKVS PAK5_Mmus APEVISRLPYGTEVDIWSLGIMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVS PAK4_Mmus APELISRLPYGPEVDIWSLGVMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKAS C45B11.1_Cele AAEVIAREPYDTRADIWSFGIMLIEMVEGEPPFFNDQPFQAMKRIRDEHEARFSRHAKVS mbt_Dmel SPEVISRLPYGPEVDIWSLGIMVIEMVDGEPPFFNEPPLQAMRRIRDMQPPNLKNAHKVS PAK4_Spur APEVISRLPYGTEVDIWSLGIMVMEMVDGEPPFFNEPPLQAMRRIRDMPPPKLKNTHKAS AqueK236_Aque APEVISKQPYGTEVDIWSLGIMVIEMIDGQPPYFNLPASEAMNCIRTLPPPTSKSPEKIS :.*:*:: * ...****:*:*::**::*:**:*. . :**. :* . . * * PAK6_Hsap PVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQ----------- PAK5_Hsap SVLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRH------------ PAK4_Hsap PSLKGFLDRLLVRDPAQRATAAELLKHPFLAKAGPPASIVPLMRQNRT------------ PAK6_Mmus PVLRDFLDRMLVREPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQ----------- PAK5_Mmus SMLRGFLDLMLVREPSQRATAQELLGHPFLKLAGPPSCIVPLMRQYRH------------ PAK4_Mmus PSLKGFLDRLLVRDPAQRATAAELLKHPFLTKAGPPASIVPLMRQHRT------------ C45B11.1_Cele VELSELLSHCIVKDVNKRWPAKDLLRHPFFAKAQHSSSIAPLLLQLQGNTINGNNPPTHH mbt_Dmel PRLQSFLDRMLVRDPAQRATAAELLAHPFLRQAGPPSLLVPLMRNARH------------ PAK4_Spur PRLLGFIERLLVRDPSQRASAFELLEHPFLRQCGPTSSLLPTMSQLRH------------ AqueK236_Aque PRLRDFLSKALVYNSEERATASELLRHPFLQFASPPSSLSKLIQFRNT------------ * ::. :* : :* .* :** ***: . : : . PAK6_Hsap ------TSTC PAK5_Hsap ---------H PAK4_Hsap ---------R PAK6_Mmus ------TSTC PAK5_Mmus ---------H PAK4_Mmus ---------R C45B11.1_Cele HSSQITTVIQ mbt_Dmel --------HP PAK4_Spur ---------K AqueK236_Aque ---------V