Gene AqueK306 (A.queenslandica)

AqueK306

Species: A.queenslandica
Alias: AqueK306
External Links:
Annotation:

Classification

Group: TK
Family: Eph

Sequence

Name Sequence Type Origin Length Description Download
AqueK306.AA Protein None 270 None Fasta, JSON
AqueK306.kin_dom Protein Kinase Domain None 223 None Fasta, JSON

Protein domain

Protein domains of AqueK306.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
Pkinase_Tyr AqueK306.AA Pkinase_Tyr 46-268 42 3.4e-103 350.12 Pfam 1-227 (270) Show / Hide 
Range on Protein: 46-268
Range on HMM: 1-227/270
Sequence Identity: 42% (99 aa)

IKVTDYLGSGQFGTVNKGVWT-TPTAGSVSVAIKTLNDNTSEDE--RVKFLQEAAIMGQF-HHPNVVKLHGVVT-IGHP--IMIVLELISGGDLKEYLNK
|.    ||.|.||.| ||.|.  |  . . ||.|.|........  | .|..||.|| |. ||||.|.|.||.|  | |  |.||.|.  .|||..|| |
ITFGKKLGEGAFGEVYKGTWKGDPDNTKIPVAVKMLKEGAMHEQNCREDFMREAKIMKQLGHHPNIVRLYGVCTDQGEPNQIYIVMEYMPHGDLLDYLRK

FKPSPGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESYYISQAKK---IPIKWTAPEALHYKKYSS
 .    |   .  .. ||. |.|||.|||||..|...||||||||.||..  ....||.|||..||.....|| .....   .||||.|||...|.|...
HR----EDKQPLTMKDLLQMCWQIAKGMEYLESKNCIHRDLAARNCLVTE--NNVVKICDFGLARDIYNDDYY-YRKRGGGKLPIKWMAPECINYGKFTT

ASDVWSYGAVMYEIWSLGHKP-FEIYTNQEVLFY
.|||||.| .|.|| ..| .| ..  .|.||. |
KSDVWSFGVLMWEIFTYGEQPTYPGMSNEEVYEY
Kinase AqueK306.AA TK 46-266 25 4.4e-93 312.37 In-house 1-414 (486) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-414/486
Sequence Identity: 25% (106 aa)

IKVTDYLGSGQFG---TVNKGVWTT----------------------PTAGSVSVAIKTLN--DNTSEDERVK-----------FLQEAAIMGQFH----
|.. . ||.||||   .| || | .                      .. | . ||.|.|   |..|..|.             ||.||..| ...    
ITLGKKLGEGQFGSFGEVYKGEWRGRNPWNGKRFPCHMTVTLKCKNGKEGGKTPVAVKMLKEGDAMSQEEFFDEEMHDRDGGEDFLEEAEEMKMMCHSLG

-HPNVVKLHGVVTIG------------HPIMIVLELISGGD----LKEYLNKFKPSPGELVLSSV-----------------------------------
 |||.|.|.||.| |            .|.|||.|. ..||    |..|| | .|   .   ..                                    
HHPNIVRLYGVCTQGEPKPCFEVVVHQEPWMIVMEYMPHGDMDGSLRDYLRKCRPKFQHEQDCPDNQHNKYPPSKLHHCMKGDITYSHESNSPCPEEQDY

-------------PSILLS-------------------LCRQIASGMEYLSRK------KFVHRDLAARNILVSDEGT---------GICKIADFGMS--
              . ||.                    |.|||.|||||..|      .||||||||||.||.....          .|||||||.|  
DDDLIKDHPPPLTTKDLLQMAWQWCLTCKHQLTMHQLLMCWQIACGMEYLHSKNCEFIQNFVHRDLAARNCLVGENNVCKWHFDGCGPVCKIADFGLSRQ

------RDL-QDESYYISQ------------------------AKKIPIKWTAPEALH---------YKKYSSASDVWSYGAVMYEIWSLG-HKPFEIYT
      ||. .|. || ..                          |.||.|.|||.|          |.|....|||||.| .|.||...|   . .  .
GSGVLARDIYSDDDYYRKSSYGTGRPMWPFEDDPWQDYYRQQSGGKLPIRWMAPECLNDRVFSEAEKYGKFTTKSDVWSFGVLMWEIFTYGQEPYYGGMS

N---------QEVL
|         |||.
NQDVWKDGDNQEVM
Kinase AqueK306.AA UTK 46-266 38 1.9e-87 296.84 In-house 1-250 (296) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-250/296
Sequence Identity: 38% (95 aa)

IKVTDYLGSGQFGTVNKGVWTTPT--AGSVSVAIKTLN-DNTSEDERV---KFLQEAA---IMGQFH-HPNVVKLHGVVTIGHPIMIVLELISGGDLKEY
..  . ||.|.||.|.|. .|...  .. . ||.|... . ....|.    .|. ||.   .|.||| ||||..| ||.| . |.|||.|. ..|||  |
VQLGRQLGQGAFGEVHKARFTQCSEHMYEMDVAVKVCQHHADAQQEPEPYPDFMEEARELQMMKQFHWHPNVLRLVGVCTQEEPWMIVTEYMENGDLRSY

LNKFKPSPGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEG-----TGICKIADFG---MSRDLQD--------ES-YYISQ-AKK
| . .|.|..  ..     .|. ...||.||.||....||||||||||.|| . .       .|||||||   |||....        || || .| ...
LRRHRPTPPACPHWLTLHQRLCMAWDIARGMQYLHSCGFVHRDLAARNCLVHMHRMMWHCMMVCKIADFGRGGMSRRVNTFHFSNRYWESYYYMAQHQDR

IPIKWTAPEALHYK-KYSSASDVWSYGAVMYEIWSLGHKPFEIYTNQEVL
.||.|.||||| .  .....|||||.| |..|||. | .|.. ..|..|.
WPIRWMAPEALTHHTRFTTKSDVWSFGVVCWEIWTWGRLPYPGMSNMQVW
Kinase AqueK306.AA Eph 46-266 46 2.5e-85 286.56 In-house 1-220 (264) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-220/264
Sequence Identity: 46% (106 aa)

IKVTDYLGSGQFGTVNKGVWTTP-TAGSV---SVAIKTLNDNTSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYLNKFKP
||..  .|.|.|| | .|   .|  .  |    |||||| .  .| .|. || ||.||||| |||...| |||| . |.||| |    | | ..| .   
IKIEKVIGAGEFGEVCCGRLKLPSGKREVWVDPVAIKTLKAGYTEKQRWDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIVTEYMENGSLDSFLRQHD-

SPGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDES----YYISQAKKIPIKWTAPEALHYKKYSSAS
  |...       |. . | ||||| |||   .||||||||||||. .   .||..|||.|| |.|..     | ..  ||||.||||||. |.|..|||
--GQFTVIQ----LVGMLRGIASGMKYLSDMNYVHRDLAARNILVNSN--LVCKVSDFGLSRVLEDDPSVEATYTTRGGKIPIRWTAPEAIAYRKFTSAS

DVWSYGAVMYEIWSLGHKPFEIYTNQEVL
|||||| || |. | | .|.   .||.|.
DVWSYGIVMWEVMSYGERPYWDMSNQDVI
Kinase AqueK306.AA SFK-Src 46-266 45 1.6e-79 235.17 In-house 1-209 (259) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-209/259
Sequence Identity: 45% (101 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLND-NTSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYLNKFKPSPG
|.    ||||||| | ||.|   |.    ||.|||    .|..|   |||||..| .  ||| ..|. | |.| || |. ||.  | |  ||    |   
IRLEKKLGSGQFGDVWKGRWNNTTE----VAVKTLKPGAMSAEE---FLQEAQVMKKLRHPNLIQLYAVCTDGEPIYIITELMKYGSLLDYLHE--PHGR

ELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESY-YIS-QAKKIPIKWTAPEALHYKKYSSASDVWSY
 | .      |  .  |.|.|| ||  . ||||||||||.|| |    .||..|||||| | ...  |   .  | |||||||||  . ..|  |||||.
SLRM----PQLVDMAAQVAHGMAYLEAQNFVHRDLAARNVLVGDN--NVCKVGDFGMSRVLAQDDEGYTAHEGAKFPIKWTAPEAMNMNRFSIKSDVWSF

GAVMYEIWSLGHKPFEIYTNQEVL
|  |.||  .| .|..  ||.|||
GILMMEIVTYGRIPYPGMTNMEVL
Kinase AqueK306.AA TyrKc 46-270 34 2e-79 271.1 SMART 1-280 (280) Show / Hide 
Range on Protein: 46-270
Range on HMM: 1-280/280
Sequence Identity: 34% (102 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLNDNTSEDERVKFLQEAAIMGQF--HHPNVVKLHGVVTIG-------HPIMIVLELISGGDLKEYL
|.  . ||.|.||.| ||.|.      . ||.|.|....    |  |..|| || ..  ||||.|.|.||.| .       .|.|||.|. ..||||.||
ITLGKKLGEGAFGEVYKGTWK------IQVAVKMLKEDA----REDFMREARIMCKLGGHHPNIVRLYGVCTQQGRNFMYIEPLMIVMEYMPHGDLKDYL

NKFKPSPGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDE-------SYYISQ---AKK--IPIKWTA
 | .|      |.  .. ||| | |||.|||||..|...||||||||.||....  ..||.|||.|||. |.       .|| ..    .|  .||.|.|
RKHRPK-----LC--MKDLLSFCWQIACGMEYLHSKNCIHRDLAARNCLVGENH--TVKISDFGLSRDIYDDDQQGESKDYYRKKEHKGGKTMLPIRWMA

PEALHYKKYSSASDVWSYGAVMYEIWSLGHKPFEI-----YTNQEVLFY-----------H-------------------------------------A
||.. |.|....|||||.| .|.|| ..| .| .       .||||. |           .                                      
PECINYGKFTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQEVIEYVKQGYRMPQPPNDYPMSFVCCPDEMYQIMKQCWHEDPEDRPTFSEIHECF
Kinase AqueK306.AA Fer 46-258 37 3.5e-79 269.25 In-house 1-220 (283) Show / Hide 
Range on Protein: 46-258
Range on HMM: 1-220/283
Sequence Identity: 37% (85 aa)

IKVTDYLGSGQFGTVNKG--VWTTPT-AGSV----SVAIKTLNDN-TSEDERVKFLQEAAIMGQ-FHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYL
.. .. ||.| ||.| ||  . .     ..|     |. |... . ...... .|..|| || . . ||||||..|| .  .|||||.|...||.|..||
VTLGKKLGEGAFGEVYKGRRTLKCKKGKWHVVNKVAVKVKKCKCEDATKEQIKEFMHEARIMRNNYDHPNVVKFYGVCVDEQPIMIVMEFCNGGSLDSYL

NKFKPSPGELVLSSVPSI--LLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGI-CKIADFGMSRDLQDESYYIS---QAKKIPIKWTAPEALHY
 | |          . |.  ..  | |.|.|||||  |. .|||.||||.|...... . |||.||||||.. |. |  .    |.|.||.|.|||.. .
KKNKNK--------TVSDNEKIRMCYQAACGMEYLHSKNCIHRDIAARNCLYHKDKVKKKCKISDFGMSRQCSDYGYDYKMKEKACKVPIRWLAPETWQT

KKYSSASDVWSYGAVMYEIWSLGHKPFE
. ||...||||.| ...|| . | .|..
GIYSPKTDVWSFGVMCWEIFTNGAEPYP
Kinase AqueK306.AA Src 46-266 42 4.2e-77 261.48 In-house 1-210 (255) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-210/255
Sequence Identity: 42% (94 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLNDNTSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYLNKFKPSPGE
||  . ||.|||| | .|.|     |.. ||.|||   |   .   ||||| || .. |...|.|. |.. . || || |. | | | .|| | ..  ..
IKLCRKLGQGQFGEVWMGYW----NGNTKVAVKTLKPGT--MSPEAFLQEAQIMKKLRHEKLVQLYAVCSQEEPIYIVTEYMSHGSLLDYLKK-DGEGRH

LVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQ--DESYYISQAKKIPIKWTAPEALHYKKYSSASDVWSYG
| |     .|...  |.|.||.||......||||||||.||...   .||.||||  | ..  |. |  ..  |.|||||||||..| ...  |||||.|
LHL----PQLIDMAAQVAEGMAYLESMNYIHRDLAARNVLVGENN--VCKVADFGLARLIKEDDDEYCARHGAKFPIKWTAPEAINYGRFTIKSDVWSFG

AVMYEIWSLGHKPFEIYTNQEVL
  ..|| . |  | .  ||.|||
ILLTEIVTYGRVPYPGMTNHEVL
Kinase AqueK306.AA TK-Unique 46-266 33 3.9e-69 236.01 In-house 1-249 (294) Show / Hide 
Range on Protein: 46-266
Range on HMM: 1-249/294
Sequence Identity: 33% (85 aa)

IKVTDYLGSGQFGTVNKGVWTTPT--AGSV-SVAIKTLNDNTSEDERVKFLQEAAIMGQFH-HPNVVKLHGVVT-IGHPIMIVLELISGGDLKEYLNKFK
..| |..|.|.||.| .|  ....   .   .||.|.|.|| .. .. .||.|...| .   |||.|.. |. |  . | ||..| ...|.|  || ...
LEVGDVIGEGAFGQVWRGEVWGIWKHWDKQKCVAVKMLKDNATDTDKQDFLKEIQMMKELGDHPNIVQCLGCCTKSDEPWMIIMEYCPCGNLQHYLRTCR

PS-----------------PGELVLSSVP-SILLSLCRQIASGMEYLSR--KKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESYYISQAK--KI
|                  | .  ....    |.. . |||.|||||..  ||.|||||.|||.|...   ..|||.||||.||....  |... .  .|
PAMRQHQTYGNLKFDFHHMPDKPRTEPISPKDLYHFAWQIACGMEYLASQRKKIVHRDLCARNVLLTE--DKVCKICDFGMARDVYTNGVYSMMCSQGRI

PIKWTAPEALH--YKKY-SSASDVWSYGAVMYEIWSLGHKPFEIYTNQEVL
||.| |||.|   .. | .. |||||.| .|.|| .||  |.  . . ...
PIRWMAPESLKCYHGMYHTTKSDVWSFGVLMWEIFTLGACPYPGMPWKDIM
Kinase AqueK306.AA Tec 46-265 41 8.7e-69 234.84 In-house 1-207 (254) Show / Hide 
Range on Protein: 46-265
Range on HMM: 1-207/254
Sequence Identity: 41% (91 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLNDNT-SEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYLNKFKPSPG
..    ||||||| |  | |.    ..  ||||...  . ||..   |..|| .|    ||. |.| || .   || || | .. | | .|| . |    
LTFMKELGSGQFGVVHLGKWR----HQYDVAIKMIKEGSMSEED---FIEEAKVMMKLSHPKLVQLYGVCSQQRPIYIVTEYMEHGCLLNYLREHK----

ELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESYYISQAKKIPIKWTAPEALHYKKYSSASDVWSYGA
   ...  .|||..| ..  ||.|| |..|.|||||||| ||.|.  .. |..||||.|   |  |  |   | |.|| .|| .|| .||| ||||..| 
-HHFQKNMAILLEMCQDVCEGMAYLERHCFIHRDLAARNCLVGDQ--CVVKVSDFGMTRYVLDDQYTSSVGTKFPVKWCPPEVFHYSRYSSKSDVWAFGV

VMYEIWSLGHKPFEIYTNQEV
 | |..|.|  |.| ..| ||
LMWEVFSCGKMPYENMSNSEV
Kinase AqueK306.AA InsR 46-268 38 6.6e-67 223.73 In-house 1-233 (278) Show / Hide 
Range on Protein: 46-268
Range on HMM: 1-233/278
Sequence Identity: 38% (91 aa)

IKVTDYLGSGQFGTVNKG--VWTTPTAGSV--SVAIKTLNDNTSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGGDLKEYLNKFKP
|     .| | ||.|  |      |  ..    .||||.|.| |  ||. ||.|| .| .| . .||.| ||.. | | ..| |.   |||| ||    |
IVLIRECGQGSFGMVYEGIAKGIVPDEEWRFGRCAIKTVNENASMRERIEFLNEASVMKEFNCHHVVRLLGVCSRGQPMYVVMEMMTYGDLKSYLRSRRP

SPG--ELVLS---SVPSI--LLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESYYISQAK-KIPIKWTAPEALHYKKY
  .  ....    .||.   ...   .|..|| ||  |||||||||.|| .|....|  .||.|||| ||. .  ||    | . |. | ||| |   ..
EEEFNNPGCNRNQHVPCLDQMHQMAAEICDGMAYLAAKKFVHRDLACRNCMVNHDFT--VKIGDFGMTRDIYEHDYYRKGGKGMLPVRWMAPESLKDGVF

SSASDVWSYGAVMYEIWSLGHKPFEIYTNQEVLFY
...|||||.| |..|. .||  |     | .|| |
TTHSDVWSFGVVLWEMVTLGEQPYQGLSNEQVLKY
Kinase AqueK306.AA Ack 46-257 39 2e-66 223.07 In-house 1-212 (266) Show / Hide 
Range on Protein: 46-257
Range on HMM: 1-212/266
Sequence Identity: 39% (87 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLNDN--TSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPI-MIVLELISGGDLKEYLNKFKPS
|..   ||.| || | .|.||.|   ...||.| | .   ..  .   ||.|.||| .  ||..|.|.|||  . |  | | ||  .| | . | |  ..
ITLCEKLGDGCFGVVWRGEWTNPGGKHIQVAVKCLRSDKMQQPNCMDDFLREVAIMHSLDHPHLVRLYGVV-LDQPMLMMVTELAPCGSLLDCLRK-HCR

PGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILV-SDEGTGICKIADFGMSRDL-QDESYYISQAKK---IPIKWTAPEALHYKKYSSAS
.  . .    ..|.  |.|||.|| ||  | ..||||||||||. ||.     ||.||| .| | | | || .. ..   .|. | |||.| ... |.||
TAFFLI----HTLCDFCMQIAMGMRYLEMKRCIHRDLAARNILLFSDD--RKVKICDFGLMRALGQNEDYYRMEFHRNLKVPFAWCAPECLRHRQFSHAS

DVWSYGAVMYEIWSLGHKPF
|||..|  . |  | | .|.
DVWMFGVTLWEMFSYGEEPW
Kinase AqueK306.AA Pkinase 46-248 25 2.3e-26 94.28 Pfam 1-204 (293) Show / Hide 
Range on Protein: 46-248
Range on HMM: 1-204/293
Sequence Identity: 25% (56 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVS-VAIKTLN-DNTSEDERVKFLQ--EAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISGG----DLKEYLN
...   .||| ||.| |...     |.   ||.|... .........  .   |..||  ..|||.|.|..... .  | .|.|.  ||    ||..|. 
YHIGRKIGSGSFGCVYKCHH----KGTGKIVAVKIIKKRHEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFEDKDHIYMVMEYCEGGLYVMDLFDYIS

KFKPSPGELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGIC----KIADFGMSRDLQDESYYISQAKKIP---IKWTAPEALH-
    . .  ..   .  . . ..||..|.||. .....||||   |||..   .| .    ||.||| .. ..   |  ..  . .   . . ||| .  
T---WRHGCFY-FSEWECKFYMYQILRGLEYCHSMGIIHRDLKPENILID--NNGHIDACVKICDFGLAKQFD---Y-NNS-MTTFCGTPWYMAPEVIRG

YKKYSSASDVWSYGAVMYE
...|....|.||.| ..||
GQYYGPKVDMWSCGCILYE
Kinase AqueK306.AA S_TKc 46-267 23 6.8e-24 86.62 SMART 1-231 (231) Show / Hide 
Range on Protein: 46-267
Range on HMM: 1-231/231
Sequence Identity: 23% (62 aa)

IKVTDYLGSGQFGTVNKGVWTTPTAGSVSVAIKTLNDNTSEDERVKFLQEAAIMGQFHHPNVVKLHGVVTIGHPIMIVLELISG--GDLKEYLNKFKPSP
 .. . .|.| ||.| |....        |||| ..         ....| .|. . ||||.|||..|    . . .|.|...|  |||..|. | .. .
YEILRKIGKGAFGKVYKCRHKK---TGRIVAIKIIK--------EHIRREIQILKK-HHPNIVKLYDVFQD-DHLYMVMEYCDGDLGDLFDYIKKRGRHG

GELVLSSVPSILLSLCRQIASGMEYLSRKKFVHRDLAARNILVSDEGTGICKIADFGMSRDLQDESYYISQAKKIPIKWTAPEALHYKKYSS-ASDVWSY
.  ...  .    ....||....||. .....||||   |||...      ||.|||..| |                . ||| |   .|.  ..|.|| 
LRFPFP--E-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE----HIKICDFGLARQL--------TTFCGTPWYMAPEVL---GYGKCKCDWWSC

GAVMYEIWSLGHKPFE---------------------IYTN----------Q-------EVLF
| ..||  . |  ||.                        .          |          |
GCILYEMLC-GYPPFPQMQMMFKKIGSPEAKDFIRKCLQKDPEKRPTAEALQDEWDIKCHPWF